Open Access

Specific cytogenetic labeling of bovine spermatozoa bearing X or Y chromosomes using fluorescent in situ hybridization (FISH)

  • François Piumi1, 3Email author,
  • Daniel Vaiman2, 3,
  • Edmond-Paul Cribiu2, 3,
  • Bernard Guérin1, 3 and
  • Patrice Humblot1, 3
Genetics Selection Evolution200133:89

DOI: 10.1186/1297-9686-33-1-89

Received: 14 August 2000

Accepted: 24 October 2000

Published: 15 January 2001

Abstract

X and Y specific probes were identified in order to apply the fluorescent in situ hybridization (FISH) technique to bovine spermatozoa. For Y chromosome detection, the BRY4a repetitive probe, covering three quarters of the chromosome, was used. For X chromosome detection, a goat Bacterial Artificial Chromosome (BAC) specific to the X chromosome of bovine and goats and giving a strong FISH signal was used. Each probe labeled roughly 45% of sperm cells. The hybridization method will be useful for evaluating the ratio of X- and Y- bearing spermatozoa in a sperm sample and consequently can be used to evaluate the efficiency of sperm sorting by different techniques such as flow cytometry.

Keywords

fluorescent in situ hybridization spermatozoon X chromosome Y chromosome bacterial artificial chromosome

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Authors’ Affiliations

(1)
UNCEIA, Services techniques
(2)
Département de génétique animale, Laboratoire de génétique biochimique et de cytogénétique, Groupe petits ruminants, Institut national de la recherche agronomique
(3)
Laboratoire de radiobiologie et d'étude du génome, Institut national de la recherche agronomique, Domaine de Vilvert

Copyright

© INRA, EDP Sciences 2001

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