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Specific cytogenetic labeling of bovine spermatozoa bearing X or Y chromosomes using fluorescent in situ hybridization (FISH)

Genetics Selection Evolution volume 33, Article number: 89 (2001) Cite this article

Abstract

X and Y specific probes were identified in order to apply the fluorescent in situ hybridization (FISH) technique to bovine spermatozoa. For Y chromosome detection, the BRY4a repetitive probe, covering three quarters of the chromosome, was used. For X chromosome detection, a goat Bacterial Artificial Chromosome (BAC) specific to the X chromosome of bovine and goats and giving a strong FISH signal was used. Each probe labeled roughly 45% of sperm cells. The hybridization method will be useful for evaluating the ratio of X- and Y- bearing spermatozoa in a sperm sample and consequently can be used to evaluate the efficiency of sperm sorting by different techniques such as flow cytometry.

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Authors and Affiliations

  1. UNCEIA, Services techniques, Maisons-Alfort, France

    François Piumi, Bernard Guérin & Patrice Humblot

  2. Département de génétique animale, Laboratoire de génétique biochimique et de cytogénétique, Groupe petits ruminants, Institut national de la recherche agronomique, 78352, Jouy-en-Josas, France

    Daniel Vaiman & Edmond-Paul Cribiu

  3. Laboratoire de radiobiologie et d'étude du génome, Institut national de la recherche agronomique, Domaine de Vilvert, 78352, Jouy-en-Josas Cedex, France

    François Piumi, Daniel Vaiman, Edmond-Paul Cribiu, Bernard Guérin & Patrice Humblot

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  1. François Piumi
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  2. Daniel Vaiman
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  3. Edmond-Paul Cribiu
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  4. Bernard Guérin
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  5. Patrice Humblot
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Correspondence to François Piumi.

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Piumi, F., Vaiman, D., Cribiu, EP. et al. Specific cytogenetic labeling of bovine spermatozoa bearing X or Y chromosomes using fluorescent in situ hybridization (FISH). Genet Sel Evol 33, 89 (2001). https://doi.org/10.1186/1297-9686-33-1-89

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  • DOI: https://doi.org/10.1186/1297-9686-33-1-89

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