Combined analysis of data from two granddaughter designs: A simple strategy for QTL confirmation and increasing experimental power in dairy cattle

  • Jörn Bennewitz1Email author,

    Affiliated with

    • Norbert Reinsch1,

      Affiliated with

      • Cécile Grohs2,

        Affiliated with

        • Hubert Levéziel2,

          Affiliated with

          • Alain Malafosse3,

            Affiliated with

            • Hauke Thomsen1,

              Affiliated with

              • Ningying Xu1,

                Affiliated with

                • Christian Looft1,

                  Affiliated with

                  • Christa Kühn4,

                    Affiliated with

                    • Gudrun A Brockmann4,

                      Affiliated with

                      • Manfred Schwerin4,

                        Affiliated with

                        • Christina Weimann5,

                          Affiliated with

                          • Stefan Hiendleder5,

                            Affiliated with

                            • Georg Erhardt5,

                              Affiliated with

                              • Ivica Medjugorac6,

                                Affiliated with

                                • Ingolf Russ6,

                                  Affiliated with

                                  • Martin Förster6,

                                    Affiliated with

                                    • Bertram Brenig7,

                                      Affiliated with

                                      • Fritz Reinhardt8,

                                        Affiliated with

                                        • Reinhard Reents8,

                                          Affiliated with

                                          • Gottfried Averdunk9,

                                            Affiliated with

                                            • Jürgen Blümel10,

                                              Affiliated with

                                              • Didier Boichard11 and

                                                Affiliated with

                                                • Ernst Kalm1

                                                  Affiliated with

                                                  Genetics Selection Evolution200335:319

                                                  DOI: 10.1186/1297-9686-35-3-319

                                                  Received: 14 June 2002

                                                  Accepted: 5 December 2002

                                                  Published: 15 May 2003

                                                  Abstract

                                                  A joint analysis of five paternal half-sib Holstein families that were part of two different granddaughter designs (ADR- or Inra-design) was carried out for five milk production traits and somatic cell score in order to conduct a QTL confirmation study and to increase the experimental power. Data were exchanged in a coded and standardised form. The combined data set (JOINT-design) consisted of on average 231 sires per grandsire. Genetic maps were calculated for 133 markers distributed over nine chromosomes. QTL analyses were performed separately for each design and each trait. The results revealed QTL for milk production on chromosome 14, for milk yield on chromosome 5, and for fat content on chromosome 19 in both the ADR- and the Inra-design (confirmed within this study). Some QTL could only be mapped in either the ADR- or in the Inra-design (not confirmed within this study). Additional QTL previously undetected in the single designs were mapped in the JOINT-design for fat yield (chromosome 19 and 26), protein yield (chromosome 26), protein content (chromosome 5), and somatic cell score (chromosome 2 and 19) with genomewide significance. This study demonstrated the potential benefits of a combined analysis of data from different granddaughter designs.

                                                  QTL mapping granddaughter design combined analysis QTL confirmation dairy cattle

                                                  (To access the full article, please see PDF)

                                                  Authors’ Affiliations

                                                  (1)
                                                  Institut für Tierzucht und Tierhaltung, Christian-Albrechts-Universität
                                                  (2)
                                                  Laboratoire de génétique biochimique et de cytogénétique, Institut national de la recherche agronomique
                                                  (3)
                                                  Union nationale des coopératives d'élevage et d'insémination animale
                                                  (4)
                                                  Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere
                                                  (5)
                                                  Institut für Tierzucht und Haustiergenetik der Justus-Liebig-Universität
                                                  (6)
                                                  Institut für Tierzucht der Ludwig-Maximilians-Universität
                                                  (7)
                                                  Institut für Veterinärmedizin der Georg-August-Universität
                                                  (8)
                                                  Vereinigte Informationssysteme Tierhaltung w.V
                                                  (9)
                                                  Bayerische Landesanstalt für Tierzucht
                                                  (10)
                                                  Institut für die Fortpflanzung landwirtschaftlicher Nutztiere
                                                  (11)
                                                  Station de génétique quantitative et appliquée, Institut national de la recherche agronomique

                                                  Copyright

                                                  © INRA, EDP Sciences 2003

                                                  Advertisement