Association with litter size of new polymorphisms on ESR1 and ESR2 genes in a Chinese-European pig line

  • Gloria Muñoz1,

    Affiliated with

    • Cristina Ovilo1,

      Affiliated with

      • Jordi Estellé2,

        Affiliated with

        • Luis Silió1Email author,

          Affiliated with

          • Almudena Fernández1 and

            Affiliated with

            • Carmen Rodriguez1

              Affiliated with

              Genetics Selection Evolution200739:195

              DOI: 10.1186/1297-9686-39-2-195

              Received: 14 July 2006

              Accepted: 31 October 2006

              Published: 17 February 2007

              Abstract

              The objective of this study was to search for polymorphisms in the coding region of the estrogen receptors 1 and 2 (ESR1 and ESR2)and to analyze the effects of these variants and the well known intronic ESR1 Pvu II polymorphism on litter size in a Chinese-European pig line. We identified five silent single nucleotide polymorphisms (SNP) in the ESR1 cDNA: c.669T > C (exon 3), c.1227C > T (exon 5), c.1452C > T (exon 7), c.1665T > C and c.1755A > G (exon 8). One pair of these SNP (c.1665T > C and c.1755A > G) co-segregated in the analyzed line, and the SNP c.669T > C showed the same segregation pattern as the Pvu II polymorphism. These polymorphisms were tested in this study, although the c.1452C > T SNP within exon 7 was not analyzed due to its low informativeness. In the ESR2 cDNA, one missense SNP was found within exon 5, which caused an amino acid substitution in the coded protein: "c.949G > A (p.Val317Met)" and was tested on sow litter size. Information on 1622 litter records from 408 genotyped sows was analyzed to determine whether these SNP influenced the total number of piglets born (TNB) or the number of born alive (NBA). The polymorphisms ESR1 : [Pvu II; c.669T > C], ESR1 : [c.1665T > C; c.1755A > G] and ESR2 : c.949G > A showed no statistically significant association with litter size. However, the ESR1 : c.1227T allele was significantly associated with TNB. The additive substitution effect was estimated to be 0.40 piglets born per litter (P < 0.03), and no dominance effects were observed. This SNP could be useful in assisted selection for litter size in some pig lines, as a new genetic marker in linkage disequilibrium with the causative mutation.

              estrogen receptor genes polymorphisms pig litter size

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              Authors’ Affiliations

              (1)
              Departamento de Mejora Genética Animal, SGIT-INIA
              (2)
              Departament de Ciència Animal i dels Aliments, UAB

              Copyright

              © INRA, EDP Sciences 2007

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