Comparative expression profiling of E. coli and S. aureus inoculated primary mammary gland cells sampled from cows with different genetic predispositions for somatic cell score

Table 4 Biological functions affected by inoculation solely

	E. coli				S. aureus
Top 5 categories of biological functions	SCS-BTA18-Q		SCS-BTA18-q		SCS-BTA18-Q
E. coli versus control SCS-BTA18-Q	p-values	Genes	p-values	Genes	p-values	Genes
Cell death	1,08E-13	96	8,30E-05	18	5,00E-05	14
Cell-to-cell signaling and interaction	3,60E-13	51	5,35E-03	12	2,45E-04	7
Hematological system development and function	3,60E-13	53	7,87E-05	14	8,09E-05	8
Immune cell trafficking	3,60E-13	34	8,62E-04	7	8,09E-05	5
Tissue development	3,60E-13	38	6,51E-03	5	3,49E-03	4
	*E. coli*				*S. aureus*
Top 5 categories of biological functions	SCS-BTA18-Q		SCS-BTA18-q		SCS-BTA18-Q
*E. coli* versus control SCS-BTA18-q	p-values	Genes	p-values	Genes	p-values	Genes
Hematological system development and function	3,60E-13	53	7,87E-05	14	8,09E-05	8
Hematopoesis	8,87E-07	27	7,87E-05	9	1,03E-04	5
Cell death	1,08E-13	96	8,30E-05	18	5,00E-05	14
Cellular development	1,67E-07	56	9,58E-05	11	1,52E-05	9
Gene expression	3,39E-11	81	1,25E-04	10	1,11E-03	10
	*E. coli*				*S. aureus*
Top 5 categories of biological functions	SCS-BTA18-Q		SCS-BTA18-q		SCS-BTA18-Q
*S. aureus* versus control SCS-BTA18-Q	p-values	Genes	p-values	Genes	p-values	Genes
Inflammatory response	4,38E-11	51	3,63E-03	11	8,30E-06	7
Cellular development	1,67E-07	56	9,58E-05	11	1,52E-05	9
Cellular growth and proliferation	9,89E-12	112	5,35E-03	18	1,52E-05	15
Tissue morphology	5,40E-05	13	6,51E-03	2	4,12E-05	3
Cell death	1,08E-13	96	8,30E-05	18	5,00E-05	15

Top 5 biological functions affected between inoculated SCS-BTA18-Q and SCS-BTA18-q cells and respective control cells; the functional category, p-values and the number of genes are shown for E. coli inoculated SCS-BTA18-Q and SCS-BTA18-q cells and S. aureus inoculated SCS-BTA18-Q cells; the categories are ranked by p-values of the SCS-BTA18-Q and SCS-BTA18-q cells, respectively and related p-values and the number of involved genes are shown for the alternative QTL allele and pathogen; for S. aureus inoculated SCS-BTA18-q cells the number of significantly differentially expressed genes was to low to perform a reliable investigation of associated molecular and cellular functions.

ISSN: 1297-9686