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  • II — Technologies de Conservation des Ressources Génétiques Animales et Végétales / Technologies of Conservation for Animal and Plant Genetic Resources
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Abscisic acid and desiccation tolerance in oil palm (Elaeis guineensis Jacq.) somatic embryos

Acide abscissique et tolerance a la dessiccation des embryons somatiques de palmier a huile (Elaeis guineensis Jacq.)

Genetics Selection Evolution volume 33, Article number: S75 (2001) Cite this article

Abstract

The effect of exogenous abscisic acid (ABA) on the desiccation tolerance, soluble sugar contents and germination rates of oil palm somatic embryos was studied. ABA was added during the development or germination of the somatic embryos. The treatments had no effect on embryo dry weights or water and sucrose contents. In contrast, monosaccharide levels decreased in ABA-treated embryos and raffinose was detected in embryos treated for 6 weeks with increasing concentrations of ABA (from 5 to 25 μM). ABA significantly improved embryo tolerance to rapid desiccation. Embryos treated with 25 μM ABA exhibited survival rates higher than that of the control. The 2-week ABA treatment induced a delay in shoot emission during embryo germination on hormone-free media. In contrast, embryos treated with 50 μM ABA displayed no germination. The presence of ABA in germination media induced inhibition of embryo shoot emission. These results suggest that ABA plays a role in the maturation and the acquisition of desiccation tolerance of oil palm somatic embryos.

Résumé

L’effet de l’acide abscissique (ABA) sur la tolerance a la dessiccation des embryons somatiques de palmier a huile, leur contenu en sucres solubles et leur taux de germination a ete etudie. L’ABA a ete ajoute pendant le developpement ou la germination des embryons somatiques. L’ABA n’a pas d’effet significatif sur le poids sec, la teneur en eau et en saccharose des embryons. En revanche, les teneurs en monosaccharides sont diminuées dans les embryons traités à l’ABA et le raffinose est détecté dans des embryons traités pendant 6 semaines avec des concentrations croissantes en ABA (de 5 à 25 μM). L’ABA améliore la tolérance des embryons à la dessiccation rapide. Les embryons traités avec 25 μM d’ABA ont un taux de survie supérieur à celui des embryons témoins. Le traitement des embryons avec 25 μM d’ABA provoque un retard dans l’émission des pousses feuillées par rapport aux témoins lors de la germination sur milieu sans hormone. En revanche, aucune germination n’est observée pour les embryons traités avec 50 μ M d’ABA. La présence d’ABA dans le milieu de germination des embryons provoque l’inhibition de l’émission des pousses feuillées. Ces résultats suggèrent un rôle de l’ABA dans la maturation et la tolérance à la dessication des embryons somatiques de palmier à huile.

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Authors and Affiliations

  1. Laboratoire GeneTrop, Centre IRD, 911, avenue Agropolis, BP 5045, 34032, Montpellier Cedex 01, France

    Frédérique Aberlenc Bertossi, Nathalie Chabrillange & Yves Duval

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  1. Frédérique Aberlenc Bertossi
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  2. Nathalie Chabrillange
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  3. Yves Duval
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Correspondence to Frédérique Aberlenc Bertossi.

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Aberlenc Bertossi, F., Chabrillange, N. & Duval, Y. Abscisic acid and desiccation tolerance in oil palm (Elaeis guineensis Jacq.) somatic embryos. Genet Sel Evol 33 (Suppl 1), S75 (2001). https://doi.org/10.1186/BF03500874

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Genetics Selection Evolution

ISSN: 1297-9686