Figure 3

Projection of detected positively selected sites (PAML/FUBAR/PRIME) and evolutionarily non-conservative sites (ConSurf) onto a 3D protein model of chicken GaGaTLR5 ECD. Positively selected sites are highlighted in red, evolutionarily non-conservative sites in blue and functionally important sites known from fish [59], and predicted for mammals [54], are in black (only positions lying in close proximity to the selected or non-conservative sites are numbered); GaGaTLR residue numbering has been adopted; selected sites or non-conservative residues located in close proximity to any of the predicted functional sites are indicated by a filled red or blue rectangle, respectively; overlap of the identified selected positions with non-conservative residues is indicated by a red number in an open blue rectangle; arrows indicate positively selected sites concurrent with those identified in previous studies (filled green = avian, filled blue = mammalian, filled green with blue border = avian and mammalian, open blue = site in neighbourhood); position 209 has been identified as both positively selected and non-conservative; in addition, this residuum (209) is also known as a flagellin-binding site in fish, as are residues 183 and 379; position 342 is both positively-selected and non-conservative and lies in close proximity to residuum 344 which has been recognised as a possible flagellin-binding site in mammals.