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Table 1 Summary of the trials to evaluate resistance to V. aestuarianus and OsHV-1 infections

From: Genetic parameters of resistance to Vibrio aestuarianus, and OsHV-1 infections in the Pacific oyster, Crassostrea gigas, at three different life stages

  Controlled disease exposure in laboratory conditions Uncontrolled disease exposure
Experiment Spat 1 Spat 2 Juv 1 Juv 2 Adult Spat 1 Spat 2 Juv 1 Juv 2 Adult Spat Spat Juvenile
Pathogen V. a. V. a. V. a. V. a. V. a. OsHV-1 OsHV-1 OsHV-1 OsHV-1 OsHV-1 Field Ponds Raceways
Date of challenge July 2013 October 2013 March 2014 June 2014 June 2015 July 2013 October 2013 April 2014 July 2014 May 2015 July 2013–September 2013 July 2013–February 2014 December 2013–May 2014
Age (months) 3 6 11 14 26 3 6 12 15 25 3 3 8
Mean individual weights (g) 0.5 2.3 6.4 12.0 34.7 0.5 2.3 6.2 11.1 37.1 0.5 0.5 8.2
Number of tanks 1 1 3 3 6 1 1 3 3 2 1 1 3
Family/tank 40 40 40 40 39 40 40 40 40 39 40 40 40
Animal/family 75 75 45 60 30 75 75 45 60 10 100 100 170
Total number of oysters tested 3000 3000 1800 2400 1170 3000 3000 1800 2400 390 4000 4000 6800
Mean mortality (range among families) (%) 5 (0–11) 15 (0–38) 49 (20–92) 44 (24–72) 89 (67–100) 86 (21–100) 74 (23–100) 59 (7–100) 41 (8–83) 55 (0–100) 89 (25–100) 56 (40–75) (62–100)
V. aestuarianus DNA detection in moribund 21/21 37/37 40/40 75/75 42/42 0/10 0/20 13/16 28/54 0/24 0/12 0/20 54/54
Mean number of copies/mL for 25 ng of total DNA 4.10E+04 2.00E+07 2.70E+07 5.60E+05 4.40E+07    2.00E+05 4.50E+02 .    4.00E+06
OsHV-1 DNA detection in moribund 0/21 0/37 0/40 12/75 10/42 10/10 20/20 16/16 54/54 23/24 12/12 0/20 16/54
Mean number of copies/µL for 25 ng of total DNA     7.00E+02 2.50E+03 2.50E+10 1.10E+07 1.70E+10 7.40E+07 7.30E+08 4.80E+09 . 6.90E+01
  1. V. a., V. aestuarianus; Juv 1 and 2, Juvenile 1 and 2; Spat, oyster <2 cm/<5 g; Juvenile, oyster 2–5 cm/5–20 g; Adult, oyster >5 cm/>20 g