Table 1 Summary of the trials to evaluate resistance to V. aestuarianus and OsHV-1 infections

Experiment

Spat 1

Spat 2

Juv 1

Juv 2

Adult

Spat 1

Spat 2

Juv 1

Juv 2

Adult

Spat

Spat

Juvenile

Pathogen

V. a.

V. a.

V. a.

V. a.

V. a.

OsHV-1

OsHV-1

OsHV-1

OsHV-1

OsHV-1

Field

Ponds

Raceways

Date of challenge

July 2013

October 2013

March 2014

June 2014

June 2015

July 2013

October 2013

April 2014

July 2014

May 2015

July 2013–September 2013

July 2013–February 2014

December 2013–May 2014

Age (months)

3

6

11

14

26

3

6

12

15

25

3

3

8

Mean individual weights (g)

0.5

2.3

6.4

12.0

34.7

0.5

2.3

6.2

11.1

37.1

0.5

0.5

8.2

Number of tanks

1

1

3

3

6

1

1

3

3

2

1

1

3

Family/tank

40

40

40

40

39

40

40

40

40

39

40

40

40

Animal/family

75

75

45

60

30

75

75

45

60

10

100

100

170

Total number of oysters tested

3000

3000

1800

2400

1170

3000

3000

1800

2400

390

4000

4000

6800

Mean mortality (range among families) (%)

5 (0–11)

15 (0–38)

49 (20–92)

44 (24–72)

89 (67–100)

86 (21–100)

74 (23–100)

59 (7–100)

41 (8–83)

55 (0–100)

89 (25–100)

56 (40–75)

(62–100)

V. aestuarianus DNA detection in moribund

21/21

37/37

40/40

75/75

42/42

0/10

0/20

13/16

28/54

0/24

0/12

0/20

54/54

Mean number of copies/mL for 25 ng of total DNA

4.10E+04

2.00E+07

2.70E+07

5.60E+05

4.40E+07

2.00E+05

4.50E+02

.

4.00E+06

OsHV-1 DNA detection in moribund

0/21

0/37

0/40

12/75

10/42

10/10

20/20

16/16

54/54

23/24

12/12

0/20

16/54

Mean number of copies/µL for 25 ng of total DNA

7.00E+02

2.50E+03

2.50E+10

1.10E+07

1.70E+10

7.40E+07

7.30E+08

4.80E+09

.

6.90E+01