Remapping of the belted phenotype in cattle on BTA3 identifies a multiplication event as the candidate causal mutation

Table 2 qPCR conditions

	prpn	lpo1	belt1	belt2	belt3	belt_b1	belt_d2
Annealing temperature (°C)	61	63	63	61	63	63	65
SYBR green	6.25	6.25	6.25	6.25	6.25	6.25	6.25
UNG	0.075	0.075	0.075	0.075	0.075	0.075	0.075
Forward (5 µM)	0.5	0.3	0.5	0.3	0.3	0.2	0.2
Reverse (5 µM)	0.5	0.5	0.5	0.5	0.5	0.3	0.2
Template	2.5	2.5	2.5	2.5	2.5	2.5	2.5
H₂O	2.675	2.875	2.675	2.875	2.875	3.175	3.275

ISSN: 1297-9686