Fig. 6
Expression of the MSRB3 protein in ear tissue samples representing different genotypes at the causative CNV. a MSRB3 immuno- and DAPI nuclear staining on ear tissue from wild-type (small-eared) and CNV/CNV (large-eared) piglets. The image was captured at FITC: 1 s, 20 X. A significant difference in MSRB3 abundance was found in both the skin and cartilage between CNV/CNV and wild-type pigs. b Western blot analysis showing MSRB3 protein levels in 18 (six CNV/CNV, six CNV/− and six −/−) pigs. The expression level of β-actin was used as loading control. c MSRB3 protein level in the 18 animals was quantified by the GeneTools software. The histogram illustrates the abundance of MSRB3 after normalization to β-actin abundance. **, P < 0.01