Fig. 1
From: Highly efficient generation of sheep with a defined FecBB mutation via adenine base editing
Evaluation of different ABE system mediated nucleotide substitutions of BMPR1B in sheep fibroblasts. a Schematic view of the target site in the sheep BMPR1B gene. sgRNA sequences are displayed in a yellow background. PAM sequences are underlined. The ABE-mediated nucleotide substitutions (g.A746G, p.Q249R) are highlighted in red. b Editing efficiency with ABE7.10, ABEmax, and xCas9-ABE in sheep fibroblasts. The editing window are displayed in a yellow background. The ABE-mediated nucleotide substitutions (g.A746G, p.Q249R) are highlighted in an orange background and red. Bystander mutations are indicated in blue. c Sanger sequencing chromatogram of intended mutations derived by the ABE system. d In sheep fibroblasts, editing efficiency with ABE7.10, ABEmax, and xCas9-ABE through deep sequencing in sheep fibroblasts. Bystander mutations are marked in blue. Three adenine base editors mediated nucleotide substitutions (g.A746G, p.Q249R) are highlighted in red