Fig. 1
From: Caprine PRNP polymorphisms N146S and Q222K are associated with proteolytic cleavage of PrPC
Goat PrPC before and after deglycosylation. Representative immunoblots of cortex homogenate (20 µg total protein) prepared from goats of different genotypes before (−) and after (+) treatment with PNGaseF. Detection of tubulin (~ 50 kDa) was used to assess sample loading. In deglycosylated samples (+), full-length PrPC is visible at ~ 26 kDa and the C1 fragment at ~ 16 kDa. Detection with anti-PrP antibodies BC6 (a), FH10 (b), F89/160.1.5 (c), and F99/97.6.1 (d) demonstrates the impact of alternative alleles on PrPC detection. Additional panels show reduced detection by BC6 in RR143 goat (a), a longer film exposure to demonstrate the faint full-length PrPC bands in samples with an allele encoding K222 (b), a shorter film exposure for NS146 goat (c), and panel in (d) shows lack of detection by F99/97.6.1 in KK222 goats even after a long film exposure. WT = II142–HH143–NN146–QQ222; HR = II142–HR143–NN146–QQ222; RR = II142–RR143–NN146–QQ222; NS = II142–HH143–NS146–QQ222; QK = II142–HH143–NN146–QK222; KK = II142–HH143–NN146–KK222