Fig. 3
From: Caprine PRNP polymorphisms N146S and Q222K are associated with proteolytic cleavage of PrPC
De-glycosylation of cellular prion protein reveals full-length PrPC and fragments C1 and C2. Representative immunoblots of cortex homogenate (20 µg total protein) from goats with PRNP genotypes that are associated with resistance to classical scrapie. Following PNGaseF treatment, SDS-PAGE, and protein transfer to PVDF, immunodetection was performed with BC6 (a) and FH10 (b); detection of tubulin served as a protein loading control. WT = II142–HH143–NN146–QQ222; NS = II142–HH143–NS146–QQ222; NSQK = II142–HH143–NS146–QK222; QK = II142–HH143–NN146–QK222; KK = II142–HH143–NN146–KK222. Sheep (ARR/ARR) homogenate added for reference in panel (a)