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Fig. 3 | Genetics Selection Evolution

Fig. 3

From: The 1.78-kb insertion in the 3′-untranslated region of RXFP2 does not segregate with horn status in sheep breeds with variable horn status

Fig. 3

Genotyping of the ancestral (anc) and derived (der) alleles in the 3′-UTR of RXFP2 by multiplex PCR. a Nucleotides at the start and end positions of the 1.78-kb insertion (derived allele) in the 3′-UTR of RXFP2. Arrows indicate the locations of the forward (F1, F2) and reverse (R1, R2) primers. b Regions and sizes of PCR products expected from multiplex PCR with primers F1, F2, R1 and R2. c Agarose gel of the PCR products obtained with primers F1, F2, R1 and R2 and DNA samples from sheep with genotypes der/der (lane 1), anc/der (lane 2) and anc/anc (lane 3). Lane 4 = DNA size marker GeneRuler™ 100 bp Plus DNA Ladder (ThermoFisher Scientific, Schwerte)

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Genetics Selection Evolution

ISSN: 1297-9686