| Controlled disease exposure in laboratory conditions | Uncontrolled disease exposure | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Experiment | Spat 1 | Spat 2 | Juv 1 | Juv 2 | Adult | Spat 1 | Spat 2 | Juv 1 | Juv 2 | Adult | Spat | Spat | Juvenile |
Pathogen | V. a. | V. a. | V. a. | V. a. | V. a. | OsHV-1 | OsHV-1 | OsHV-1 | OsHV-1 | OsHV-1 | Field | Ponds | Raceways |
Date of challenge | July 2013 | October 2013 | March 2014 | June 2014 | June 2015 | July 2013 | October 2013 | April 2014 | July 2014 | May 2015 | July 2013–September 2013 | July 2013–February 2014 | December 2013–May 2014 |
Age (months) | 3 | 6 | 11 | 14 | 26 | 3 | 6 | 12 | 15 | 25 | 3 | 3 | 8 |
Mean individual weights (g) | 0.5 | 2.3 | 6.4 | 12.0 | 34.7 | 0.5 | 2.3 | 6.2 | 11.1 | 37.1 | 0.5 | 0.5 | 8.2 |
Number of tanks | 1 | 1 | 3 | 3 | 6 | 1 | 1 | 3 | 3 | 2 | 1 | 1 | 3 |
Family/tank | 40 | 40 | 40 | 40 | 39 | 40 | 40 | 40 | 40 | 39 | 40 | 40 | 40 |
Animal/family | 75 | 75 | 45 | 60 | 30 | 75 | 75 | 45 | 60 | 10 | 100 | 100 | 170 |
Total number of oysters tested | 3000 | 3000 | 1800 | 2400 | 1170 | 3000 | 3000 | 1800 | 2400 | 390 | 4000 | 4000 | 6800 |
Mean mortality (range among families) (%) | 5 (0–11) | 15 (0–38) | 49 (20–92) | 44 (24–72) | 89 (67–100) | 86 (21–100) | 74 (23–100) | 59 (7–100) | 41 (8–83) | 55 (0–100) | 89 (25–100) | 56 (40–75) | (62–100) |
V. aestuarianus DNA detection in moribund | 21/21 | 37/37 | 40/40 | 75/75 | 42/42 | 0/10 | 0/20 | 13/16 | 28/54 | 0/24 | 0/12 | 0/20 | 54/54 |
Mean number of copies/mL for 25Â ng of total DNA | 4.10E+04 | 2.00E+07 | 2.70E+07 | 5.60E+05 | 4.40E+07 | Â | Â | 2.00E+05 | 4.50E+02 | . | Â | Â | 4.00E+06 |
OsHV-1 DNA detection in moribund | 0/21 | 0/37 | 0/40 | 12/75 | 10/42 | 10/10 | 20/20 | 16/16 | 54/54 | 23/24 | 12/12 | 0/20 | 16/54 |
Mean number of copies/µL for 25 ng of total DNA |  |  |  | 7.00E+02 | 2.50E+03 | 2.50E+10 | 1.10E+07 | 1.70E+10 | 7.40E+07 | 7.30E+08 | 4.80E+09 | . | 6.90E+01 |