Fig. 2

Determination of the sufficient number of oligonucleotides and oligonucleotide density and genomic coverage. a Hybridization of the test oligo pool on SSC13 showing insufficient signal intensity when oligo density or probe coverage (band widths) is reduced (the ATTO 425 signal merged with the background signal making it undetectable); and b schematic representations of test results (left) and optimized probe design (right). Dashes indicate minimal oligo density, lines indicate intermediate density, and solid colour indicates full density. Colour height (quarter, half, or full) represents genomic coverage. c final oligo-banding pattern of SSC13 showing strong signals from fluorophores 6-FAM, ATTO 550, and ATTO 647N. Oligo density and coverage were sufficient for the detection of chromosomal rearrangements. Probes 4 and 5 appear as a single band in this image because they were designed very close to each other. Scale bar = 1 µm