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Fig. 7 | Genetics Selection Evolution

Fig. 7

From: Comparative analyses of dynamic transcriptome profiles highlight key response genes and dominant isoforms for muscle development and growth in chicken

Fig. 7

Switching of dominant transcripts of TNFRSF6B affects skeletal muscle development between the AA and LS breeds. a qPCR detection of the overexpression of the two isoforms in chicken primary myoblasts (CPM) for 24 h. Effects of the overexpression of the two isoforms on the proliferation of CPMs as determined by flow cytometry (b) and CCK-8 assay (c, d) Relative expression of genes involved in cell proliferation after overexpression of the two isoforms in CPM. e Detection of novel cells after overexpression of the two isoforms via ethynyl-deoxyuridine assay, respectively. f The number of novel cells after overexpression of the two isoforms, respectively. g Relative expression of genes involved in cell differentiation after overexpression of the two isoforms in CPM. h Immunofluorescence detection of differentiated CPM. Red represents differentiated myotubes, and blue represents cell nuclei. i Relative protein content after overexpression of the two isoforms in the CPM. * Represents p < 0.05, ** represents p < 0.01, *** represents p < 0.001

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Genetics Selection Evolution

ISSN: 1297-9686