Fig. 6

Refining eRNA-mediated gene regulatory networks (eGRN) by self-transcribing active regulatory region sequencing (STARR-Seq) and subsequent identification of functional genes in adipocyte differentiation. a The workflow for STARR-seq strategy to detect SNPs influencing DNA activity. b Regulatory SNPs identified by STARR-seq that affect enhancer activity. c Refined eGRN underlying fat-related traits identified through regulatory SNP screening. d siRNA knockdown effects of five potential target genes on adipocyte differentiation assessed by Oil Red O Staining in 3T3-L1 pre-adipocytes. NC represents the blank control. e siRNA knockdown effects of potential target genes on marker gene expression during adipocyte differentiation in 3T3-L1 pre-adipocytes. (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001)