Open Access

A repetitive probe for FISH analysis of bovine interphase nuclei

  • Wafa Slimane1, 2,
  • Daniel Vaiman3,
  • Sophie Godard3,
  • Anne Vaiman3,
  • Edmond Cribiu3 and
  • Jean-Paul Renard1Email author
Genetics Selection Evolution200032:217

https://doi.org/10.1186/1297-9686-32-2-217

Received: 22 June 1999

Accepted: 20 December 1999

Published: 15 March 2000

Abstract

The purpose of this study was to generate repetitive DNA sequence probes for the analysis of interphase nuclei by fluorescent in situ hybridisation (FISH). Such probes are useful for the diagnosis of chromosomal abnormalities in bovine preimplanted embryos. Of the seven probes (E1A, E4A, Ba, H1A, W18, W22, W5) that were generated and partially sequenced, five corresponded to previously described Bos taurus repetitive DNA (E1A, E4A, Ba, W18, W5), one probe (W22) shared no homology with other DNA sequences and one (H1A) displayed a significant homology with Rattus norvegicus mRNA for secretin receptor transmembrane domain 3. Fluorescent in situ hybridisation was performed on metaphase bovine fibroblast cells and showed that five of the seven probes hybridised most centromeres (E1A, E4A, Ba, W18, W22), one labelled the arms of all chromosomes (W5) and the H1A probe was specific to three chromosomes (ch14, ch20, and ch25). Moreover, FISH with H1A resulted in interpretable signals on interphase nuclei in 88% of the cases, while the other probes yielded only dispersed overlapping signals.

Keywords

satellite DNA FISH bovine centromere

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Authors’ Affiliations

(1)
Unité biologie du développement, Institut national de la recherche agronomique
(2)
Services techniques de l'Union national des coopératives d'élevage et d'insémination artificielle
(3)
Génétique biochimique et cytogénétique, Institut national de la recherche agronomique

Copyright

© INRA, EDP Sciences 2000

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