Open Access

A high-density linkage map of the RN region in pigs

  • Christian Looft1Email author,
  • Denis Milan2,
  • Jin-Tae Jeon3,
  • Sven Paul1,
  • Norbert Reinsch1,
  • Claire Rogel-Gaillard4,
  • Virginie Rey2,
  • Valérie Amarger3,
  • Annie Robic2,
  • Ernst Kalm1,
  • Patrick Chardon4 and
  • Leif Andersson3
Genetics Selection Evolution200032:321

https://doi.org/10.1186/1297-9686-32-3-321

Received: 6 December 1999

Accepted: 10 February 2000

Published: 15 May 2000

Abstract

The porcine RN locus affects muscle glycogen content and meat quality. We previously mapped the RN locus to chromosome 15. This study describes the identification of polymorphisms for four class I and four class II markers located in the RN region. Resource families were genotyped with F-SSCP markers (fluorescent single strand conformation polymorphism) and microsatellite markers. Subsequent multipoint linkage analysis revealed the order FN1-IGFBP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906. The gene order is identical to the previously reported porcine RH map of the same region. The described map will facilitate positional cloning of the RN gene.

Keywords

pigmeat qualityRN genegenetic mapping

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Authors’ Affiliations

(1)
Institute of Animal Breeding and Husbandry, Christian-Albrechts-University
(2)
Laboratoire de génétique cellulaire, Institut national de la recherche agronomique
(3)
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences
(4)
Laboratoire de radiobiologie et d'étude du génome, Institut national de la recherche agronomique/Centre énergie atomique

Copyright

© INRA, EDP Sciences 2000

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