Open Access

Cytogenetical anchoring of sheep linkage map and syntenic groups using a sheep BAC library

  • Kamila Tabet-Aoul1, 2,
  • Anne Oustry-Vaiman2,
  • Daniel Vaiman2,
  • Nadhira Saïdi-Mehtar3,
  • Edmond-Paul Cribiu2Email author and
  • Frédéric Lantier1
Genetics Selection Evolution200032:441

https://doi.org/10.1186/1297-9686-32-4-441

Received: 7 September 1999

Accepted: 14 March 2000

Published: 15 July 2000

Abstract

In order to simultaneously integrate linkage and syntenic groups to the ovine chromosomal map, a sheep bacterial artificial chromosome (BAC) library was screened with previously assigned microsatellites using a sheep-hamster hybrid panel and genetic linkage. Thirty-three BACs were obtained, fluorescently labelled and hybridised on sheep-goat hybrid metaphases (2n = 57). This study allowed us, (i), to anchor all linkage groups on sheep chromosomes, (ii), to give information on the probable position of the centromere on the linkage map for the centromeric chromosomes, (iii), to contradict the previous orientation of the ovine × linkage group by the mapping of BMS1008 on OARXq38. Concerning our somatic cell hybrid panel, this study resulted in the assignment of all the previously unassigned groups to ovine chromosomes and a complete characterisation of the hybrid panel. In addition, since hybridisations were performed on a sheep-goat hybrid, new marker/anchoring points were added to the caprine cytogenetic map.

Keywords

mapping microsatellite sheep BAC library FISH

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Authors’ Affiliations

(1)
Laboratoire de pathologie infectieuse et immunologie, Institut national de la recherche agronomique
(2)
Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Institut national de la recherche agronomique
(3)
Laboratoire de biologie moléculaire et génétique, Université Oran Es-Sénia

Copyright

© INRA, EDP Sciences 2000

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