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Cytogenetical anchoring of sheep linkage map and syntenic groups using a sheep BAC library

  • 1, 2,
  • 2,
  • 2,
  • 3,
  • 2Email author and
  • 1
Genetics Selection Evolution200032:441

https://doi.org/10.1186/1297-9686-32-4-441

  • Received: 7 September 1999
  • Accepted: 14 March 2000
  • Published:

Abstract

In order to simultaneously integrate linkage and syntenic groups to the ovine chromosomal map, a sheep bacterial artificial chromosome (BAC) library was screened with previously assigned microsatellites using a sheep-hamster hybrid panel and genetic linkage. Thirty-three BACs were obtained, fluorescently labelled and hybridised on sheep-goat hybrid metaphases (2n = 57). This study allowed us, (i), to anchor all linkage groups on sheep chromosomes, (ii), to give information on the probable position of the centromere on the linkage map for the centromeric chromosomes, (iii), to contradict the previous orientation of the ovine × linkage group by the mapping of BMS1008 on OARXq38. Concerning our somatic cell hybrid panel, this study resulted in the assignment of all the previously unassigned groups to ovine chromosomes and a complete characterisation of the hybrid panel. In addition, since hybridisations were performed on a sheep-goat hybrid, new marker/anchoring points were added to the caprine cytogenetic map.

Keywords

  • mapping
  • microsatellite
  • sheep
  • BAC library
  • FISH

(To access the full article, please see PDF)

Authors’ Affiliations

(1)
Laboratoire de pathologie infectieuse et immunologie, Institut national de la recherche agronomique, 37380 Nouzilly, France
(2)
Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Institut national de la recherche agronomique, 78352 Jouy-en-Josas Cedex, France
(3)
Laboratoire de biologie moléculaire et génétique, Université Oran Es-Sénia, 31000 Oran, Algérie

Copyright

© INRA, EDP Sciences 2000

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