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A first genotyping assay of French cattle breeds based on a new allele of the extension gene encoding the melanocortin-1 receptor (Mc1r)

  • 1,
  • 1,
  • 1,
  • 1,
  • 3,
  • 3,
  • 4,
  • 1, 2,
  • 1 and
  • 1Email author
Genetics Selection Evolution200032:511

https://doi.org/10.1186/1297-9686-32-5-511

  • Received: 21 February 2000
  • Accepted: 6 June 2000
  • Published:

Abstract

The seven transmembrane domain melanocortin-1 receptor (Mc1r) encoded by the coat color extension gene (E) plays a key role in the signaling pathway of melanin synthesis. Upon the binding of agonist (melanocortin hormone, α-MSH) or antagonist (Agouti protein) ligands, the melanosomal synthesis of eumelanin and/or phaeomelanin pigments is stimulated or inhibited, respectively. Different alleles of the extension gene were cloned from unrelated animals belonging to French cattle breeds and sequenced. The wild type E allele was mainly present in Normande cattle, the dominant ED allele in animals with black color (i.e. Holstein), whereas the recessive e allele was identified in homozygous animals exhibiting a more or less strong red coat color (Blonde d'Aquitaine, Charolaise, Limousine and Salers). A new allele, named E1, was found in either homozygous (E1/E1) or heterozygous (E1/E) individuals in Aubrac and Gasconne breeds. This allele displayed a 4 amino acid duplication (12 nucleotides) located within the third cytoplasmic loop of the receptor, a region known to interact with G proteins. A first genotyping assay of the main French cattle breeds is described based on these four extension alleles.

Keywords

  • cattle
  • genotyping
  • coat color
  • extension
  • polymorphism

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Authors’ Affiliations

(1)
Unité de génétique moléculaire animale, UMR 1061 (Université/INRA), Université de Limoges, 87060 Limoges Cedex, France
(2)
Unité de génétique biochimique et cytogénétique, UR339, Institut national de la recherche agronomique, 78352 Jouy-en-Josas Cedex, France
(3)
Laboratoire d'analyses génétiques pour les espèces animales (LABOGENA), Institut national de la recherche agronomique, 78352 Jouy-en-Josas Cedex, France
(4)
Station de génétique quantitative et appliquée, UR337, Institut national de la recherche agronomique, 78352 Jouy-en-Josas Cedex, France

Copyright

© INRA, EDP Sciences 2000

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