Open Access

Isolation of subtelomeric DNA sequences labelling sheep and goat chromosome ends

  • Daniel Vaiman1,
  • Ana Brunialti1,
  • Mohamed Bensaada2,
  • Céline Derbois1,
  • Anne Vaiman1,
  • Allan Crawford3,
  • Philippe Metezeau2 and
  • Edmond P Cribiu1Email author
Genetics Selection Evolution200032:599

https://doi.org/10.1186/1297-9686-32-6-599

Received: 3 May 2000

Accepted: 15 September 2000

Published: 15 November 2000

Abstract

Two techniques that make it possible to isolate telomere DNA are presented, using sheep as an example. The first technique is based upon the screening of a sheep BAC library with PCR amplified DNA segments preserved from high-power laser beam irradiation. Twenty-three BACs hybridising to 13 subtelomeric regions in sheep and goats were obtained (out of 27 in the sheep complement), of which 13 recognised more than one region, telomeric or not. Twenty-three microsatellites were isolated from these BACs and 22 were genetically mapped on the sheep international genetic map, always consistently with the cytogenetical localisation in 17 cases out of 22. These results are discussed. The second technique is based upon the selective cloning of subtelomeric enriched DNA. Preliminary results were obtained by this approach.

Keywords

telomeresFISHsheepgoats

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Authors’ Affiliations

(1)
Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Institut national de la recherche agronomique
(2)
Laboratoire de cytométrie analytique, Institut Pasteur
(3)
AgResearch Molecular Biology Unit, University of Otago

Copyright

© INRA, EDP Sciences 2000

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