Open Access

Functional study and regional mapping of 44 hormono-regulated genes isolated from a porcine granulosa cell library

  • Gwenola Tosser-Klopp1Email author,
  • Agnès Bonnet1,
  • Martine Yerle1 and
  • François Hatey1
Genetics Selection Evolution200133:69

https://doi.org/10.1186/1297-9686-33-1-69

Received: 12 May 2000

Accepted: 31 August 2000

Published: 15 January 2001

Abstract

cDNA clones from a pig granulosa cell cDNA library were isolated by differential hybridisation for follicle stimulating hormone (FSH) regulation in granulosa cells in a previous study. The clones that did not match any known sequence were studied for their expression in granulosa cells (treated or not by FSH) and in fresh isolated ovarian follicles mainly by comparative RT-PCR analysis. These results give functional data on genes that may be implicated in follicular growing. These ESTs have been localised on the porcine genome, using a somatic cell hybrid panel, providing new type I markers on the porcine map and information on the comparative map between humans and pigs.

Keywords

pig ovarian follicle cDNA mapping comparative RT-PCR comparative map

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Authors’ Affiliations

(1)
Laboratoire de génétique cellulaire, Institut national de la recherche agronomique

Copyright

© INRA, EDP Sciences 2001

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