Open Access

A higher resolution radiation hybrid map of bovine chromosome 13

  • Jörg Schläpfer1Email author,
  • Nasikhat Stahlberger-Saitbekova1,
  • Sergio Comincini2,
  • Claude Gaillard1,
  • David Hills3,
  • Rudolf K Meyer4,
  • John L Williams3,
  • Jim E Womack5,
  • Andreas Zurbriggen4 and
  • Gaudenz Dolf1
Genetics Selection Evolution200234:255

https://doi.org/10.1186/1297-9686-34-2-255

Received: 25 June 2001

Accepted: 7 December 2001

Published: 15 March 2002

Abstract

In this paper, we present a radiation hybrid framework map of BTA13 composed of nine microsatellite loci, six genes and one EST. The map has been developed using a recently constructed 12'000 rad bovine-hamster whole-genome radiation hybrid panel. Moreover, we present a comprehensive map of BTA13 comprising 72 loci, of which 45 are microsatellites, 20 are genes and seven are ESTs. The map has an estimated length of 2694.7 cR12'000. The proposed order is in general agreement with published maps of BTA13. Our results only partially support previously published information of five blocks of conserved gene order between cattle and man. We found no evidence for the existence of an HSA20 homologous segment of coding DNA on BTA13 located centromeric of a confirmed HSA10 homologous region. The present map increases the marker density and the marker resolution on BTA13 and enables further insight into the evolutionary development of the chromosome as compared to man.

Keywords

bovine chromosome 13 radiation hybrid gene mapping 12'000 rad comparative mapping

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Authors’ Affiliations

(1)
Institute of Animal Genetics, Nutrition and Housing, Faculty of Veterinary Medicine, University of Berne
(2)
Department of Genetics and Microbiology, University of Pavia
(3)
Division of Genomics and Bioinformatics, Roslin Institute (Edinburgh), Roslin
(4)
Institute of Animal Neurology, Faculty of Veterinary Medicine, University of Berne
(5)
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University

Copyright

© INRA, EDP Sciences 2002

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