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Application of disease-associated differentially expressed genes – Mining for functional candidate genes for mastitis resistance in cattle

  • 1Email author,
  • 1,
  • 1,
  • 2,
  • 2,
  • 3,
  • 3,
  • 3 and
  • 1
Genetics Selection Evolution200335 (Suppl 1) :S19

  • Accepted: 4 February 2003
  • Published:


In this study the mRNA differential display method was applied to identify mastitis-associated expressed DNA sequences based on different expression patterns in mammary gland samples of non-infected and infected udder quarters of a cow. In total, 704 different cDNA bands were displayed in both udder samples. Five hundred-and-thirty two bands, (75.6%) were differentially displayed. Ninety prominent cDNA bands were isolated, re-amplified, cloned and sequenced resulting in 87 different sequences. Amongst the 19 expressed sequence tags showing a similarity with previously described genes, the majority of these sequences exhibited homology to protein kinase encoding genes (26.3%), to genes involved in the regulation of gene expression (26.3%), to growth and differentiation factor encoding genes (21.0%) and to immune response or inflammation marker encoding genes (21.0%). These sequences were shown to have mastitis-associated expression in the udder samples of animals with and without clinical mastitis by quantitative RT-PCR. They were mapped physically using a bovine-hamster somatic cell hybrid panel and a 5000 rad bovine whole genome radiation hybrid panel. According to their localization in QTL regions based on an established integrated marker/gene-map and their disease-associated expression, four genes (AHCY, PRKDC, HNRPU, OSTF1) were suggested as potentially involved in mastitis defense.


  • mastitis
  • expressed sequence tag
  • gene expression
  • cattle
  • RH mapping

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Authors’ Affiliations

Research Unit for Molecular Biology, Research Institute for the Biology of Farm Animals, Dummerstorf, Germany
Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843, USA
Department of Animal Genetics, University of Warmia and Mazury, Olsztyn, Poland


© INRA, EDP Sciences 2003