Volume 35 Supplement 1

Second International Symposium on Candidate Genes for Animal Health. 16-18 August 2002, Montpelier - France

Open Access

Application of disease-associated differentially expressed genes – Mining for functional candidate genes for mastitis resistance in cattle

  • Manfred Schwerin1Email author,
  • Diana Czernek-Schäfer1,
  • Tom Goldammer1,
  • Srinivas R. Kata2,
  • James E. Womack2,
  • Ravi Pareek3,
  • Chandra Pareek3,
  • Krzysztof Walawski3 and
  • Ronald M. Brunner1
Genetics Selection Evolution200335(Suppl 1):S19

https://doi.org/10.1186/1297-9686-35-S1-S19

Accepted: 4 February 2003

Published: 15 June 2003

Abstract

In this study the mRNA differential display method was applied to identify mastitis-associated expressed DNA sequences based on different expression patterns in mammary gland samples of non-infected and infected udder quarters of a cow. In total, 704 different cDNA bands were displayed in both udder samples. Five hundred-and-thirty two bands, (75.6%) were differentially displayed. Ninety prominent cDNA bands were isolated, re-amplified, cloned and sequenced resulting in 87 different sequences. Amongst the 19 expressed sequence tags showing a similarity with previously described genes, the majority of these sequences exhibited homology to protein kinase encoding genes (26.3%), to genes involved in the regulation of gene expression (26.3%), to growth and differentiation factor encoding genes (21.0%) and to immune response or inflammation marker encoding genes (21.0%). These sequences were shown to have mastitis-associated expression in the udder samples of animals with and without clinical mastitis by quantitative RT-PCR. They were mapped physically using a bovine-hamster somatic cell hybrid panel and a 5000 rad bovine whole genome radiation hybrid panel. According to their localization in QTL regions based on an established integrated marker/gene-map and their disease-associated expression, four genes (AHCY, PRKDC, HNRPU, OSTF1) were suggested as potentially involved in mastitis defense.

Keywords

mastitis expressed sequence tag gene expression cattle RH mapping

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Authors’ Affiliations

(1)
Research Unit for Molecular Biology, Research Institute for the Biology of Farm Animals
(2)
Department of Veterinary Pathobiology, Texas A&M University
(3)
Department of Animal Genetics, University of Warmia and Mazury

Copyright

© INRA, EDP Sciences 2003

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