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  • Research
  • Open Access

A first generation bovine BAC-based physical map

  • 1Email author,
  • 1,
  • 1,
  • 2,
  • 1,
  • 1,
  • 3,
  • 1 and
  • 1
Genetics Selection Evolution200436:105

https://doi.org/10.1186/1297-9686-36-1-105

  • Received: 18 June 2003
  • Accepted: 17 October 2003
  • Published:

Abstract

A first generation clone-based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site (STS) marker screening. The BAC clones were selected from an Inra BAC library (105 984 clones) and a part of the CHORI-240 BAC library (26 500 clones). The contigs were anchored using the screening information for a total of 1303 markers (451 microsatellites, 471 genes, 127 EST, and 254 BAC ends). The final map, which consists of 6615 contigs assembled from 100 923 clones, will be a valuable tool for genomic research in ruminants, including targeted marker production, positional cloning or targeted sequencing of regions of specific interest.

Keywords

  • cattle
  • physical map
  • BAC

(To access the full article, please see PDF)

Authors’ Affiliations

(1)
Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Institut national de la recherche agronomique, Centre de recherche de Jouy, 78352 Jouy-en-Josas Cedex, France
(2)
Laboratoire de radiobiologie et d'étude du génome, Département de génétique animale, Institut national de la recherche agronomique, Centre de recherche de Jouy, 78352 Jouy-en-Josas Cedex, France
(3)
Station de génétique quantitative appliquée, Département de génétique animale, Institut national de la recherche agronomique, Centre de recherche de Jouy, 78352 Jouy-en-Josas Cedex, France

Copyright

© INRA, EDP Sciences 2004

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