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Genetics Selection Evolution

Open Access

Comparative analysis of vertebrate EIF2AK2 (PKR) genes and assignment of the equine gene to ECA15q24–q25 and the bovine gene to BTA11q12–q15

  • Andrey A. Perelygin1Email author,
  • Teri L. Lear2,
  • Andrey A. Zharkikh3 and
  • Margo A. Brinton1
Genetics Selection Evolution200638:551

Received: 19 January 2006

Accepted: 13 April 2006

Published: 6 September 2006


The structures of the canine, rabbit, bovine and equine EIF2AK2 genes were determined. Each of these genes has a 5' non-coding exon as well as 15 coding exons. All of the canine, bovine and equine EIF2AK2 introns have consensus donor and acceptor splice sites. In the equine EIF2AK2 gene, a unique single nucleotide polymorphism that encoded a Tyr329Cys substitution was detected. Regulatory elements predicted in the promoter region were conserved in ungulates, primates, rodents, Afrotheria (elephant) and Insectifora (shrew). Western clawed frog and fugu EIF2AK2 gene sequences were detected in the USCS Genome Browser and compared to those of other vertebrate EIF2AK2 genes. A comparison of EIF2AK2 protein domains in vertebrates indicates that the kinase catalytic domains were evolutionarily more conserved than the nucleic acid-binding motifs. Nucleotide substitution rates were uniform among the vertebrate sequences with the exception of the zebrafish and goldfish EIF2AK2 genes, which showed substitution rates about 20% higher than those of other vertebrates. FISH was used to physically assign the horse and cattle genes to chromosome locations, ECA15q24–q25 and BTA11q12–15, respectively. Comparative mapping data confirmed conservation of synteny between ungulates, humans and rodents.


translation initiation factorinnate immunityphylogenetic analysisFISH mapping

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Authors’ Affiliations

Biology Department, Georgia State University, Atlanta, USA
Department of Veterinary Science, M.H. Gluck Equine Research Center, University of Kentucky, Lexington, USA
Bioinformatics Department, Myriad Genetics, Inc, Salt Lake City, USA


© INRA, EDP Sciences 2006