Open Access

Cloning, chromosome mapping and expression pattern of porcine PLIN and M6PRBP1 genes

  • Xia Tao1,
  • Yuan Jihong1,
  • Gan Li1,
  • Feng Bin1,
  • Zhu Yi1,
  • Chen Xiaodong1,
  • Zhang Peichao1 and
  • Zaiqing Yang1Email author
Genetics Selection Evolution200840:215

https://doi.org/10.1186/1297-9686-40-2-215

Received: 4 December 2006

Accepted: 31 August 2007

Published: 15 March 2008

Abstract

The PAT proteins, named after the three PLIN/ADRP/TIP47 (PAT) proteins, PLIN for perilipin, ADRP for adipose differentiation-related protein and TIP47 for tail-interacting protein of 47 kDa, now officially named M6PRBP1 for mannose-6-phosphate receptor binding protein 1, is a set of intracellular lipid droplet binding proteins. They are localized in the outer membrane monolayer enveloping lipid droplets and are involved in the metabolism of intracellular lipid. This work describes the cloning and sequencing of porcine PLIN and M6PRBP1 cDNAs, the chromosome mapping of these two genes, as well as the expression pattern of porcine PAT genes. Sequence analysis shows that the porcine PLIN cDNA contains an open reading frame of 1551 bp encoding 516 amino acids and that the porcine M6PRBP1 cDNA contains a coding region of 1320 bp encoding 439 amino acids. Comparison of PLIN and M6PRBP1 amino-acid sequences among various species reveals that porcine and bovine proteins are the most conserved. Porcine PLIN and M6PRBP1 genes have been mapped to pig chromosomes 7 and 2, respectively, by radiation hybrid analysis using the IMpRH panel. Expression analyses in pig showed a high expression of PLIN mRNA in adipose tissue, M6PRBP1 mRNA in small intestine, kidney and spleen and ADRP mRNA in adipose tissue, lung and spleen.

Keywords

pig PLIN M6PRBP1 cDNA cloningchromosome mappingtissue expression pattern

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Authors’ Affiliations

(1)
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University

Copyright

© INRA, EDP Sciences 2008

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