Table 1 Sequences of adapters and primers used in the AFLP detection
From: Duck (Anas platyrhynchos) linkage mapping by AFLP fingerprinting
Name | Sequence | |
|---|---|---|
Adapter Eco RI | ||
Eco Top Strand | 5-CTCGTAGACTGCGTACC | |
Eco Bottom Strand | 5-AATTGGTACGCAGTCTAC | |
Adapter Taq I | ||
Taq Top Strand | 5-GACGATGAGTCCTGAC | |
Taq Bottom Strand | 5-CGGTCAGGACTCAT | |
Primer Eco RI | ||
Eco R+A | 5-GAC TGC GTA CCG TAC CA | |
E1 | VIC-Eco R+AAA | 5-GAC TGC GTA CCG TAC CAA A |
E2 | NED-Eco R+AAC | 5-GAC TGC GTA CCG TAC CAA C |
E3 | PET-Eco R+AAG | 5-GAC TGC GTA CCG TAC CAA G |
E4 | FAM-Eco R+ACA | 5-GAC TGC GTA CCG TAC CAC A |
E5 | VIC-Eco R+AC | 5-GAC TGC GTA CCG TAC CAC |
E6 | FAM-Eco R+AG | 5-GAC TGC GTA CCG TAC CAG |
Primer Taq I | ||
Taq+A | 5-GAT GAG TCC TGA CCG AA | |
T1 | Taq+AAC | 5-GAT GAG TCC TGA CCG AAA C |
T2 | Taq+AAG | 5-GAT GAG TCC TGA CCG AAA G |
T3 | Taq+AAT | 5-GAT GAG TCC TGA CCG AAA T |
T4 | Taq+ACA | 5-GAT GAG TCC TGA CCG AAC A |
T5 | Taq+AC | 5-GAT GAG TCC TGA CCG AAC |
T6 | Taq+AG | 5-GAT GAG TCC TGA CCG AAG |