Table 2 Conditions of selective amplification PCR
From: Duck (Anas platyrhynchos) linkage mapping by AFLP fingerprinting
Hold | Cycle | Number of cycles | ||
|---|---|---|---|---|
94°C, 5 min | 94°C, 30 s | 66°C, 30 s | 72°C, 1 min | 2 |
- | 94°C, 30 s | 64°C, 30 s | 72°C, 1 min | 2 |
- | 94°C, 30 s | 62°C, 30 s | 72°C, 1 min | 2 |
- | 94°C, 30 s | 60°C, 30 s | 72°C, 1 min | 2 |
- | 94°C, 30 s | 58°C, 30 s | 72°C, 1 min | 2 |
- | 94°C, 30 s | 56°C, 30 s | 72°C, 1 min | 25 |
- | - | - | 72°C, 5 min | 1 |
4°C, forever | - | - | - | 1 |