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Table 5 Molecular and cellular functions affected by inoculation and cell culture

From: Comparative expression profiling of E. coli and S. aureus inoculated primary mammary gland cells sampled from cows with different genetic predispositions for somatic cell score

  E. coli inoculated Un-inoculated control
Top 5 categories of molecular and cellular functions SCS-BTA18-Q SCS-BTA18-q SCS-BTA18-Q SCS-BTA18-q
E. coli inoculated
SCS-BTA18-Q cells
p-values Genes p-values Genes p-values Genes p-values Genes
Cell cycle 2,98E-18 120 2,26E-25 164 1,98E-04 25 1,19E-02 2
Cellular growth and proliferation 1,10E-10 215 2,56E-11 279 1,28E-02 10 3,28E-02 2
Cellular assembly and organization 9,30E-10 59 2,14E-10 70 1,28E-02 12 1,33E-03 2
DNA replication, recombination and repair 9,30E-10 96 2,14E-10 154 3,18E-02 5 --- ---
RNA-post-transcriptional modification 3,70E-06 39 9,50E-06 42 1,58E-03 11 4,56E-02 1
  E. coli inoculated Un-inoculated control
Top 5 categories of molecular and cellular functions SCS-BTA18-Q SCS-BTA18-q SCS-BTA18-Q SCS-BTA18-q
E. coli inoculated
SCS-BTA18-q cells
p-values Genes p-values Genes p-values Genes p-values Genes
Cell cycle 2,98E-18 120 2,26E-25 164 1,98E-04 25 1,19E-02 2
Cellular growth and proliferation 1,10E-10 215 2,56E-11 279 1,28E-02 10 3,28E-02 2
Cellular assembly and organization 9,30E-10 59 2,14E-10 70 1,28E-02 12 1,33E-03 2
DNA replication, recombination and repair 9,30E-10 96 2,14E-10 154 3,18E-02 5 --- ---
Cell death 7,33E-06 153 2,27E-10 227 6,73E-03 8 --- ---
  S. aureus inoculated E. coli inoculated Un-inoculated control
Top 5 categories of molecular and cellular functions SCS-BTA18-Q SCS-BTA18-Q SCS-BTA18-q SCS-BTA18-Q
S. aureus inoculated
SCS-BTA18-Q cells
p-values Genes p-values Genes p-values Genes p-values Genes
Cellular assembly and organization 6,54E-05 14 9,30E-10 59 2,14E-10 70 1,28E-02 12
Cell death 2,03E-04 33 7,33E-06 153 2,27E-10 227 6,73E-03 8
DNA replication, recombination and repair 2,61E-04 20 9,30E-10 96 2,14E-10 154 3,18E-02 5
Nucleic acid metabolism 2,61E-04 9 3,54E-03 4 4,61E-03 14 7,60E-04 6
Small molecule biochemistry 2,61E-04 18 7,60E-05 53 3,80E-03 26 2,56E-04 19
  1. Top five molecular and cellular functions affected between time points 1 h and 24 h in SCS-BTA18-Q and SCS-BTA18-q cells by inoculation; the molecular and cellular functional category, p-values and the number of involved genes are shown for E. coli inoculated SCS-BTA18-Q and SCS-BTA18-q cells and S. aureus inoculated SCS-BTA18-Q cells, as well as for the control cells; the categories are ranked by p-values of the SCS-BTA18-Q and SCS-BTA18-q cells, respectively, and related p-values and the number of involved genes are shown for the alternative QTL allele and the un-inoculated control cells; for S. aureus inoculated SCS-BTA18-q cells the number of significantly differentially expressed genes was to low to perform a reliable investigation of associated molecular and cellular functions; hence, for SCS-BTA18-Q cells additionally the related p-values and the number of involved genes are shown for E. coli inoculated cells and for un-inoculated SCS-BTA18-Q control cells.