Table 1 Primers used in this study
Primers purpose | Primer name | Primer sequence (5'-3') | Product size (bp) | Tm (Ā°C) |
|---|---|---|---|---|
RT-PCR | VLDLR-F | ATGGCCAGGATCGTAGACTT | 292 | 58.0 |
| Ā | VLDLR-R | TCATTTATCTGAGGAGCAGG | Ā | Ā |
3'- RACE | GSP-3F | ATATTTGAGGACCGTGTGTACTGGA | 1280 | 68.0 |
3'- Nested | NGSP-3F | ACTGGATCTGAATTGGTTACCCT | Ā | 60.0 |
5'-RACE | GSP-5R | TGCATCATTGAGGTTGTTTACTAGG | Ā | 68.0 |
5'-Nested | NGSP-5R1 | ACTGCTTCATTCTCTCCATCAATCC | 1006 | 60.0 |
| Ā | NGSP-5R2 | GCATTCATTTATGTTGCATTCCT | 742 | 62.0 |
| Ā | NGSP-5R3 | TTTCACCATCACATTTCCAGGAC | 590 | 65.0 |
Expression profile | VLDLR-A | AAAGTATACCTGTGCATGTCC | 268/178 | 60.0 |
| Ā | VLDLR-S | CATGAAGTAGCCAGCCACC | Ā | Ā |
Internal control | Ī²-actin-A | AACTGGGATGACATGGAGAAGA | 104 | 60.0 |
| Ā | Ī²-actin-S | GGGTTCAGGGGAGCCTCTGT | Ā | Ā |
| Ā | VLDLR-F1 | TGTTCCTTCCTCATCCTCTT | Ā | Ā |
Polymorphism | VLDLR-R1 | CAGAACAAACTCATAGCTACC | 315 | 56.5 |
| Ā | VLDLR-F2 | CAGGACATGCACAGGTATACTT | 168 | 56.0 |
| Ā | VLDLR-R2 | TACCTCTGGAGCATGAAGGCTCAC | Ā | Ā |