Table 3 Real-time PCR primer combinations
Primer name | Gene ID | Species | Primer | Primer sequence (5’- 3’) |
|---|---|---|---|---|
GAPDH | Z19086 | C. coturnix | Forward | GGCACTGTCAAGGCTGAGAAT |
Reverse | GCATCTCCCCACTTGATGTTG | |||
HMGCR | NM_204485 | G. gallus | Forward | GCAGAGGGCCTTACAAC |
Reverse | GGAGGAGCAAGCCGTAT | |||
FDFT1 | NM_001039294 | G. gallus | Forward | GCCATCATGTACCAGTATGTG GAA |
Reverse | GCTGCGTCTTGTTGGAGGAA | |||
SQLE | NM_001030953 | G. gallus | Forward | GAGGTAGAAATTCCTTTTCCAACATCT |
Reverse | GCCGTGATGGAAGGACCTT | |||
DHCR7 | XM_420914 | G. gallus | Forward | GGGAAAGATTGGAAACGCTACA |
Reverse | CAGATTCTGTGTCAGCCTTAAAACA | |||
ABCG5 | XM_419457 | G. gallus | Forward | ATTACAAGATCCCAAGGTCATGCT |
Reverse | GAGACGATCTGGTTTGCAGTCA | |||
ABCG8 | XM_419458 | G. gallus | Forward | GCCTTCCAGCATGTTTTTCAG |
Reverse | CGCAACCGTAGCTCTGCTATT | |||
APOA1 | D85133 | C. coturnix | Forward | TCTGGTGCAGGAATTCAAGGA |
| Â | Â | Â | Reverse | TCATCCAGGAGGTCGATCAAG |