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  • III — Méthodes D’inventaire et de Caractérisation de la Diversité Génétique en Milieu Naturel / Methods for Inventory and Characterization of Genetic Diversity in Natural Environment
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Development of microsatellite multiplexes for wild goats using primers designed from domestic Bovidae

Développement de multiplexes microsatellites pour les Capra sauvages à partir d’amorces définies chez les Bovidea domestiques

Genetics Selection Evolution volume 33, Article number: S193 (2001) Cite this article

Abstract

Many wild goat taxa (Capra spp.) are endangered and would benefit from the availability of molecular tools that are useful for population management and conservation. We developed microsatellite DNA markers useful in all wild goat species, by using a cross-species amplification approach. Seventy-five microsatellite primer pairs designed from domestic cattle (Bos taurus), sheep (Ovis aries) and goat (Capra hircus) were tested on three distantly related Capra species: C. ibex ibex, C. [i.] sibirica, and C. pyrenaica. On average, 90% of the domestic ungulate primers amplified a microsatellite PCR product in the wild goat species. Forty percent of the total were polymorphic in C. i. ibex, which is expected to have the lowest genetic diversity among all Capra species. We developed multiplexes of 24 polymorphic fluorescent microsatellite loci that can be amplified in 13 PCR reactions and loaded into two gel-lanes. These microsatellites will allow studies of conservation and population ecology in all Capra species, and the multiplexes will reduce the time and cost of the genetic analyses.

Résumé

Plusieurs espèces sauvages du genre Capra sont considérées comme menacées et requièrent un outil moléculaire fiable pour les études de gestion des populations et de conservation. Un ensemble de marqueurs microsatellites utilisable pour l’ensemble des espèces du genre Capra a été développé en employant l’approche de transfert inter-spécifique d’amorces microsatellite. Soixante-quinze couples d’amorces microsatellite définies sur les bovins (Bos taurus), ovins (Ovis aries) et caprins (Capra hircus) domestiques ont été testés sur trois espèces génétiquement distantes du genre Capra: C. ibex ibex, C. [i.] sibirica, and C. pyrenaica. Quatre-vingt dix pour-cent de ces amorces ont conduit à l’obtention d’un produit d’amplification spécifique. Quarante pour cent de ces marqueurs se sont révélés polymorphes chez Capra. i. ibex, espèce estimée la moins variable de l’ensemble du genre Capra. Un système de multiplexes a été développé afin d’amplifier 24 loci polymorphes au cours de 13 réactions PCR indépendantes, et de les charger dans deux pistes de séquenceur automatique. Ces microsatellites donnent la possibilité d’études de conservation et d’écologie sur l’ensemble des espèces du genre Capra, et les multiplexes développés permettent une réduction du co du temps de ces analyses génétiques.

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Authors and Affiliations

  1. Laboratoire de biologie des populations d’altitude (Cnrs - UMR 5553), Université Joseph Fourier, BP. 53, 38041, Grenoble Cedex 9, France

    Célia Maudet, Gordon Luikart & Pierre Taberlet

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  1. Célia Maudet
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  2. Gordon Luikart
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  3. Pierre Taberlet
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Correspondence to Célia Maudet.

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Maudet, C., Luikart, G. & Taberlet, P. Development of microsatellite multiplexes for wild goats using primers designed from domestic Bovidae. Genet Sel Evol 33 (Suppl 1), S193 (2001). https://doi.org/10.1186/BF03500880

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Genetics Selection Evolution

ISSN: 1297-9686