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  • III — Méthodes D’inventaire et de Caractérisation de la Diversité Génétique en Milieu Naturel / Methods for Inventory and Characterization of Genetic Diversity in Natural Environment
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Original article Microsatellite analysis of polyandry and spawning site competition in brown trout (Salmo trutta L.)

Analyse de la polyandrie et de la compétition pour les sites de reproduction chez la truite commune (Salmo trutta L.) à l’aide de marqueurs microsatellites

Genetics Selection Evolution volume 33, Article number: S205 (2001) Cite this article

Abstract

Polyandry and competition for spawning sites in a sedentary brown trout population was studied using four highly variable microsatellite loci. All loci combined gave average exclusion probabilities of 0.91 and 0.97 based on two reference samples of spawners. All eggs from 22 redds of this population were collected. For 10 of these redds, tissue samples of potential mothers, i.e. females that were observed to show spawning behaviour on these redds, were sampled. Only in four cases, the observed four-locus genotype of the putative mother conformed with corresponding egg-genotypes. For all four redds polyandry was detected encompassing two fathering males. We applied a sequential procedure of multiple paternity analysis that allowed the assignment of a single paternal genotype to each individual egg, and thus, to quantify the genetic contribution of different males. In each redd, one male fertilised the majority of eggs (60-92%) and the contribution of additional males ranged between 8-40%. The total number of eggs in the four redds represented 11-21% of the total fecundity of the mothers. This indicates that females in this population spread their eggs among several redds. Eight cases of incomplete redd superimposition were detected among all 22 redds analysed indicating strong competition for spawning sites between females.

Résumé

Une étude de la polyandrie et de la compétition pour les

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Authors and Affiliations

  1. Institut national de la recherche agronomique, Laboratoire de génétique des poissons, 78352, Jouy-en-Josas Cedex, France

    Carlo R. Largiadèr, Arnaud Estoup, Frédéric Lecerf & René Guyomard

  2. Division of Population Biology, Institute of Zoology, University of Berne, Baltzerstrasse 6, 3012, Berne, Switzerland

    Carlo R. Largiadèr

  3. Institut national de la recherche agronomique, Station d’hydrobiologie lacustre, 75 avenue de Corzent, 74203, Thonon-les-Bains, France

    Alexis Champigneulle

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  1. Carlo R. Largiadèr
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Correspondence to Carlo R. Largiadèr.

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Largiadèr, C.R., Estoup, A., Lecerf, F. et al. Original article Microsatellite analysis of polyandry and spawning site competition in brown trout (Salmo trutta L.). Genet Sel Evol 33 (Suppl 1), S205 (2001). https://doi.org/10.1186/BF03500881

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Genetics Selection Evolution

ISSN: 1297-9686