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  • III — Méthodes D’inventaire et de Caractérisation de la Diversité Génétique en Milieu Naturel / Methods for Inventory and Characterization of Genetic Diversity in Natural Environment
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Use of RFLP markers to study genetic diversity and to build a core-collection of the wild wheat relative Ae. geniculata Roth (= Ae. ovata L.)

Utilisation de marqueurs RFLP pour l’étude de la diversité génétique et l’établissement d’une core collection d’Ae. geniculata Roth (= Ae. ovata L.)

Genetics Selection Evolution volume 33, Article number: S269 (2001) Cite this article

Abstract

The wild wheat relative Ae. geniculata Roth (= Ae. ovata L.) is a preferentially seifing, allo-tetraploid species (MU genome) widely distributed around the Mediterranean Basin. Two hundred and two individuals belonging to one hundred and fifty one populations originating from different eco-geographical regions were used to study its genetic diversity. The diversity was revealed on Restriction Fragment Length Polymorphisms (RFLP). Nine clones mapped on the Triticeae group 2 chromosome consensus map were used as probes. The genetic variability was found to be highly structured. A fine scale study performed on populations from Bulgaria, France and Morocco suggested that 52% of the diversity was explained by differences among populations within regions. A Factorial Analysis of Correspondence (FAC) distinguished two main structures corresponding mainly to the North and South of the Mediterranean Sea. Differences among close regions separated by natural barriers were also found. Hypotheses concerning the dissemination of Ae. geniculata around the Mediterranean Sea are presented and examined. In order to improve the management of Ae. geniculata genetic resources, a methodology for core collection sampling is presented and the efficiency of RFLP markers in building this collection is discussed.

Résumé

Ae. geniculata Roth (= Ae. ovata L.) est une espèce apparentée au blé, allotétraploïde (de génome MU), à l’autogamie prépondérante, largement distribuée sur le pourtour du Bassin Méditerranéen. Deux cent deux individus, appartenant à cent cinquante et une populations originaires de différentes régions éco-géographiques, ont été utilisés pour étudier la diversité génétique de l’espèce. L’étude a été basée sur le polymorphisme de longueur des fragments de restriction (Restriction Fragments Length Polymorphism, RFLP). Neuf clones, cartographiés sur le chromosome 2 de la carte consensus des Triticeae, ont été utilisés comme sondes. La variabilité génétique de cette espèce s’est révélée très structurée entre populations. Une étude à petite échelle, réalisée sur des populations de Bulgarie, France et Maroc, a suggéré que 52% de la diversité s’expliquait par des différences entre populations à l’intérieur des régions. Une Analyse Factorielle des Correspondances (AFC) a permis de distinguer deux structures principales, correspondant au nord et au sud de la Méditerranée. Des différences ont également été notées entre régions proches séparées par des barrières naturelles. Des hypothèses concernant la diffusion d’ie. geniculata autour de la Méditerranée sont présentées et examinées. Afin d’améliorer la gestion des ressources génétiques d’Ae. geniculata, une méthodologie d’établissement d’une core-collection est étudiée, et l’efficacité des marqueurs RFLP pour la construction de cette collection est discutée.

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Authors and Affiliations

  1. UMR Diversité et génome des plantes cultivées, Ensam-Inra, 34060, Montpellier Cedex, France

    Maria Zaharleva & Sylvain Santoni

  2. UMR Diversité et génome des plantes cultivées, Institut national de la recherche agronomique, 34130, Mauguio, France

    Jacques David

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  1. Maria Zaharleva
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Correspondence to Jacques David.

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Zaharleva, M., Santoni, S. & David, J. Use of RFLP markers to study genetic diversity and to build a core-collection of the wild wheat relative Ae. geniculata Roth (= Ae. ovata L.). Genet Sel Evol 33 (Suppl 1), S269 (2001). https://doi.org/10.1186/BF03500884

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Genetics Selection Evolution

ISSN: 1297-9686