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  • III — Méthodes D’inventaire et de Caractérisation de la Diversité Génétique en Milieu Naturel / Methods for Inventory and Characterization of Genetic Diversity in Natural Environment
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Caractérisation de la diversité d’une population de vignes sauvages du Pic Saint-Loup (Hérault) et relations avec le compartiment cultivé

Characterization of genetic diversity in a population of wild grapes from Pic Saint Loup area and it’s relationship with the cultivated grapes

Genetics Selection Evolution volume 33, Article number: S289 (2001) Cite this article

Résumé

Vitis vinifera est la seule espèce endémique de vigne en Europe. Le compartiment sauvage Vitis vinifera subspecies silvestris a subi une forte érosion et seuls quelques peuplements subsistent encore actuellement. Afin de mieux connaître les relations entre les compartiments sauvage et cultivé, nous avons engagé une étude de la diversité de populations naturelles. Une population de vignes sauvages du Pic Saint-Loup (Hérault) a ainsi été caractérisée. La cartographie de cette population constituée d’une quinzaine d’individus ainsi que quelques caractéristiques biologiques sont présentées. Le caractère dïoique de la plupart des accessions a été confirmé avec une présence importante de pieds mâles (58 %). La diversité des individus est discutée et comparée à l’aide de différentes approches: étude morphométrique des feuilles (ampélométrie), polymorphisme moléculaire à l’aide de dix locus microsatellites, degré de tolérance à l’oïdium (Uncinula necator) et au phylloxera. Dans le cas des marqueurs moléculaires, les données sont comparées aux résultats obtenus sur un échantillon incluant les principaux cépages cultivés et autochtones présents dans la région. L’analyse en composantes principales réalisée à partir de ces données fait apparaître une séparation très nette des individus sauvages par rapport aux individus cultivés.

Abstract

Vitis vinifera is in fact the only species of grapevine endemic in Europe. The wild compartment Vitis vinifera subspecies silvestris suffers of important erosion and just a few populations are still subsisting today. To better know the relations between the wild and the cultivated compartment we began a study about the diversity of the natural populations. For that, a group of about fifteen wild vines being situated on the Pic Saint-Loup (Hérault, France) has been characterized. The mapping of the population and some biological characteristics are presented. We verified that most of the accessions owned effectively flowers showing separated sex. The majority of the vines (58%) were male. The diversity inside the population is discussed and compared through different approaches. We analyzed the morphology of the accessions by ampelography and measurement of the leaves. The molecular polymorphism of the individuals has been evaluated with ten microsatellites loci and we checked the degree of tolerance of the accessions to powdery mildiou (Uncinula necator) and to phylloxera. With the molecular markers we also compared the data of the wild population to the results obtained on a sample of old varieties originated from the area and of the actual main cultivated varieties. The principal component analysis based on these molecular data shows finally a very clear division between the wild accessions and the cultivated varieties.

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Authors and Affiliations

  1. Institut national de la recherche agronomique - ENS A.M., 2, place P. Viala, 34060, Montpellier Cedex 1, France

    Patrice This, Catherine Roux, Pierre Parra, René Siret, Thibaut Bourse, Anne-Françoise Adam, Michel Yvon, Thierry Lacombe, Jacques David & Jean-Michel Boursiquot

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  1. Patrice This
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  2. Catherine Roux
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  3. Pierre Parra
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  4. René Siret
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  5. Thibaut Bourse
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  6. Anne-Françoise Adam
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  7. Michel Yvon
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  8. Thierry Lacombe
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  9. Jacques David
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  10. Jean-Michel Boursiquot
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Correspondence to Patrice This.

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This, P., Roux, C., Parra, P. et al. Caractérisation de la diversité d’une population de vignes sauvages du Pic Saint-Loup (Hérault) et relations avec le compartiment cultivé. Genet Sel Evol 33 (Suppl 1), S289 (2001). https://doi.org/10.1186/BF03500885

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Genetics Selection Evolution

ISSN: 1297-9686