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  • IV — Inventaire, Caractérisation et Suivi de la Diversité Microbienne / Inventory, Characterization and Monitoring of Microbial Diversity
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Evaluation of AFLP for the grouping of Bradyrhizobium strains

Évaluation de l’AFLP pour le groupage des souches de Bradyrhizobium

Genetics Selection Evolution volume 33, Article number: S365 (2001) Cite this article

Abstract

In recent years we have characterized 250 Bradyrhizobium strains, mainly from Senegal, using several taxonomic techniques i.e. numerical taxonomy of pheno-typic features, Biolog system, SDS-PAGE of total cellular proteins, 16S rDNA RFLP and sequence analyses, 16S-23S rDNA intergenic gene spacer RFLP and sequence analyses, AFLP, DNA:DNA hybridizations. Here we evaluate the taxonomic resolving power of these techniques by comparing the results obtained on various subsets of the same strains. We conclude that AFLP is a useful method for an initial grouping of Bradyrhizobium strains and provides infraspecific information. However, from a limited comparison, it appears that the less labor-intensive 16S-23S rDNA intergenic gene spacer analysis gives similar results and may provide additional information on deeper groupings.

Résumé

Au cours des dernières années, nous avons caractérisé 250 souches de Bradyrhizobium, principalement du Sénégal, par différentes techniques, i.e., analyse numérique de caractères phénotypiques, analyse Biolog, SDS-PAGE des protéines cellulaires totales, RFLP et séquençage du gène codant pour l’ARN ribosomique 16S ou de l’espace intergénique entre les gènes ribosomiques 16S et 23S, AFLP et hybridations ADN:ADN. Nous évaluons ici le niveau de résolution taxonomique de ces différentes techniques par comparaison des résultats obtenus sur différentes sélections des mêmes souches. L’AFLP s’avère être une technique efficace pour le groupement initial des souches de Bradyrhizobium qui renseigne au niveau infraspécifique. Cependant l’analyse de l’intergène 16S-23S, moins lourd à mettre en œuvre, apparaît plus performant en permettant un groupement plus fin.

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Authors and Affiliations

  1. Laboratorium voor Microbiologie, Faculteit Wetenschappen, Universiteit Gent, K.L., Ledeganckstraat 35, B-9000, Gent, Belgium

    Anne Willems & Monique Gillis

  2. Laboratoire des symbioses tropicales et méditerranéennes, UMR 113 Inra/AGRO-M/Cirad/IRD, TA 10 / J, Campus International de Baillarguet, 34398, Montpellier Cedex 5, France

    Florence Doignon-Bourcier & Philippe de Lajudie

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  1. Anne Willems
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  2. Florence Doignon-Bourcier
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  3. Monique Gillis
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  4. Philippe de Lajudie
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Correspondence to Philippe de Lajudie.

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Willems, A., Doignon-Bourcier, F., Gillis, M. et al. Evaluation of AFLP for the grouping of Bradyrhizobium strains. Genet Sel Evol 33 (Suppl 1), S365 (2001). https://doi.org/10.1186/BF03500890

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Genetics Selection Evolution

ISSN: 1297-9686