Figure 2

Alignment between ASIP protein sequences from 10 vertebrate species. ASIP amino acid sequences from various species were collected from Ensembl (mouse: ENSMUST00000109697; horse: ENSECAT00000004772; cow: ENSBTAT00000048322; sheep: ENSOART00000010128; dog: ENSCAFT00000038625; cat: ENSFCAT00000011040; human: ENST00000568305; chicken: ENSGALT00000044768; zebrafish: ENSDART00000113083). ASIP sequences are identified with the name of the species on the left. The mouse ASIP sequence (reference sequence) is at the top of the alignment. Non-conserved residues in the 10 species analyzed are shown in grey. Conserved residues are indicated in black within the reference sequence and represented by black dots in other sequences. Dashes represent deletions. The conserved C117 residue in donkey ASIP that corresponds to the murine C113 residue is circled. The 10 functional cysteine residues that have been shown to be involved in the activity of ASIP are indicated with stars. Non-agouti mutations identified in other domestic mammalian species are reported above the corresponding position. Dog and alpaca mutations consist in missense mutations and a 19 amino acids (aa) in-frame deletion. Rabbit, rat, cat and horse mutations are frameshifts. Mouse and sheep regulatory mutations are not shown.