Fig. 2
From: Highly efficient generation of sheep with a defined FecBB mutation via adenine base editing
Detection of ABEmax-mediated nucleotide substitutions in founder animals. a Genotypes of target sites by TA cloning in all founder animals. Bystander mutations are highlighted in blue. The ABEmax-mediated nucleotide substitutions (g.A746G, p.Q249R) are highlighted in red. b Genotypes of target sites through deep sequencing in eight founder animals. Bystander mutations are marked in blue. The ABEmax-mediated nucleotide substitutions (g.A746G, p.Q249R) are highlighted in red. c Mutation rate at the targeted region in eight founder animals