Fig. 4

Mammary RNA-seq alignments representing ABO intron/exon 5 splicing structures of the chr11:104242578GG and chr11:104242578CC genotypes. The site of the proposed chr11:104242578C > G essential splice donor SNP is indicated, with individual reads and coverage data showing alternate splice forms in the animal carrying the mutation. This coverage track also represents the cryptic, ‘ + 33 bp long splice’ transcript as the minority splice form relative to the ‘− 8 bp short splice’ transcript, the former representing an in-frame variant, with the latter causing a predicted frameshifted protein isoform