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Fig. 3 | Genetics Selection Evolution

Fig. 3

From: Integration of multi-omics data reveals cis-regulatory variants that are associated with phenotypic differentiation of eastern from western pigs

Fig. 3

Differential promoter variants of the LYZ gene might contribute to high roughage digestibility and gastro-intestinal immunity in Luchuan. a Haplotypes of LYZ promoter variants across all eastern and western pigs. b Comparing the expression level of LYZ between the Luchuan and Duroc breeds by qPCR. c Comparing the protein level of LYZ between Luchuan and Duroc. d Luciferase reporter assays comparing the promoter activity of promoter sequences from Luchuan (pGL3-Luchuan), from Duroc (pGL3-Duroc), and from Duroc modified with the Luchuan promoter allele (one indel and two adjacent SNPs) (pGL3-Duroc-modified). e Comparing the expression level of LYZ before and after knockdown of NR2F2. f Frequencies of the Luchuan promoter allele (two adjacent SNPs and one indel) across different pig breeds. DUR: Duroc pig; LW: Large White pig; LD: Landrace pig; YM: Yucatan miniature pig; YNT: Tibetan pig (Yunnan); TC: Tongcheng pig; TT: Tibetan pig (Qinghai-Tibetan); HTDE: Hetaodaer pig; SCT: Tibetan pig (Sichuan); WZS: Wuzhishan pig; BMX: Bamaxiang pig; EHL: Erhualian pig; LUC: Luchuan pig; LWH: Laiwu black pig; MIN: Min pig (Shandong). g A model of how the genetic differentiation of the LYZ promoter might contribute to the adaption of Luchuan to a less clean environment and plant-enriched roughage feeding. The sequence divergences between Luchuan and Duroc are shaded in light yellow

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Genetics Selection Evolution

ISSN: 1297-9686