Fig. 3
From: Mutation of the MYH3 gene causes recessive cleft palate in Limousine cattle
Identification of the cleft palate mutations in the MYH3 gene. a Results of homozygosity mapping on BTA19 on the ARS-UCD1.2 reference genome. b Number of discordant animals among the 16 affected animals. The region of homozygosity is defined by the homozygous haplotype on BTA19 shared by all affected animals, from bp 29,396,440 to 30,048,251. c Electropherograms of a wild-type and a cleft palate-affected animal after PCR amplification with primers MYH3ex24F and MYH3ex24R. Since MYH3 is located on the reverse strand, mutations are referred to respectively as T/C for the substitution and CTTGAGCTGACA/A for the deletion to match with the transcription sense. Cds coding sequence, aa amino-acid, WT wild-type, CP cleft palate. d Scheme presenting the MYH3 protein domains and the predicted consequences of the mutations on the MYH3 protein. IQ calmodulin binding domain, WT wild-type, CP cleft palate